Selection and validation of experimental condition-specific reference genes for qRT-PCR in Metopolophium dirhodum (Walker) (Hemiptera: Aphididae)
نویسندگان
چکیده
منابع مشابه
نمونهبرداری دنبالهای شتهی Metopolophium dirhodum (Hemiptera: Aphididae) در مزارع گندم
این پژوهش به منظور تهیهی الگوی نمونهبرداری دنبالهای شتهی گندم-گل سرخ،Metopolophium dirhodum (Walker)، در مزارع گندم آبی منطقهی جلیلآباد ورامین انجام شد. برای این منظور نمونهبرداریهای هفتگی از جمعیت شته در طول دو فصل زراعی 1379 و 1380 انجام شد. در هر نمونهبرداری، تعداد 50 ساقهی گندم به طور تصادفی انتخاب و تعداد شتههای موجود شمارش شد. با دادههای بهدست آمده، الگوی توزیع فضایی جمعیت ش...
متن کاملSelection and Validation of Reference Genes for qRT-PCR in Cycas elongata
Quantitative reverse transcription PCR (qRT-PCR) is a sensitive technique used in gene expression studies. To achieve a reliable quantification of transcripts, appropriate reference genes are required for comparison of transcripts in different samples. However, few reference genes are available for non-model taxa, and to date, reliable reference genes in Cycas elongata have not been well charac...
متن کاملSelection and Validation of Endogenous Reference Genes for qRT-PCR Analysis in Leafy Spurge (Euphorbia esula)
Quantitative real-time polymerase chain reaction (qRT-PCR) is the most important tool in measuring levels of gene expression due to its accuracy, specificity, and sensitivity. However, the accuracy of qRT-PCR analysis strongly depends on transcript normalization using stably expressed reference genes. The aim of this study was to find internal reference genes for qRT-PCR analysis in various exp...
متن کاملSelection and Validation of Appropriate Reference Genes for qRT-PCR Analysis in Isatis indigotica Fort.
Due to its sensitivity and specificity, real-time quantitative PCR (qRT-PCR) is a popular technique for investigating gene expression levels in plants. Based on the Minimum Information for Publication of Real-Time Quantitative PCR Experiments (MIQE) guidelines, it is necessary to select and validate putative appropriate reference genes for qRT-PCR normalization. In the current study, three algo...
متن کاملIdentification and Validation of Reference Genes for the Normalization of Gene Expression Data in qRT-PCR Analysis in Aphis gossypii (Hemiptera: Aphididae)
To obtain accurate and reliable results from quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) analysis, it is necessary to select suitable reference genes as standards for normalizing target gene expression data. QRT-PCR is a popular analytical methodology for studying gene expression and it has been used widely in studies of Aphis gossypii Glover in recent years...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Scientific Reports
سال: 2020
ISSN: 2045-2322
DOI: 10.1038/s41598-020-78974-z